Using a bioaerosol personal sampler in combination with real‐time PCR analysis for rapid detection of airborne viruses
Identifieur interne : 001F75 ( Main/Exploration ); précédent : 001F74; suivant : 001F76Using a bioaerosol personal sampler in combination with real‐time PCR analysis for rapid detection of airborne viruses
Auteurs : Oleg V. Pyankov [Australie] ; Igor E. Agranovski [Australie] ; Olga Pyankova [Australie] ; Ekaterina Mokhonova [Australie] ; Vlad Mokhonov [Australie] ; Alexander S. Safatov [Russie] ; Alexander A. Khromykh [Australie]Source :
- Environmental Microbiology [ 1462-2912 ] ; 2007-04.
Abstract
We have recently developed a new personal sampler and demonstrated its feasibility for detection of viable airborne microorganisms including bacteria, fungi and viruses. To accelerate the time‐consuming analytical procedure involving 2–5 days of biological testing, we employed a real‐time PCR protocol in conjunction with the personal sampler for collection of airborne viruses. The advantage of this approach is that if the presence of a particular pathogen in the air is detected by the PCR, the remaining collecting liquid can be further analysed by more time‐consuming biological methods to estimate the number of airborne infectious/live microorganisms. As sampling of bioaerosols in natural environments is likely to be associated with substantial contamination by a range of microorganisms commonly existing in an ambient air, an investigation of the specificity of detection by targeted PCR analysis is required. Here we present the results of the study on the detection of Influenza virus in the ambient air contaminated with high concentrations of bacteria and fungi using real‐time PCR protocol. The combined sampling PCR detection method was found to be fully feasible for the rapid (∼2.5 h) and highly specific (no cross‐reactivity) identification of the labile airborne virus in the air containing elevated concentrations of other microorganisms.
Url:
DOI: 10.1111/j.1462-2920.2006.01226.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000832
- to stream Istex, to step Curation: 000827
- to stream Istex, to step Checkpoint: 000C52
- to stream Main, to step Merge: 001F76
- to stream Main, to step Curation: 001F75
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Using a bioaerosol personal sampler in combination with real‐time PCR analysis for rapid detection of airborne viruses</title><author><name sortKey="Pyankov, Oleg V" sort="Pyankov, Oleg V" uniqKey="Pyankov O" first="Oleg V." last="Pyankov">Oleg V. Pyankov</name></author><author><name sortKey="Agranovski, Igor E" sort="Agranovski, Igor E" uniqKey="Agranovski I" first="Igor E." last="Agranovski">Igor E. Agranovski</name></author><author><name sortKey="Pyankova, Olga" sort="Pyankova, Olga" uniqKey="Pyankova O" first="Olga" last="Pyankova">Olga Pyankova</name></author><author><name sortKey="Mokhonova, Ekaterina" sort="Mokhonova, Ekaterina" uniqKey="Mokhonova E" first="Ekaterina" last="Mokhonova">Ekaterina Mokhonova</name></author><author><name sortKey="Mokhonov, Vlad" sort="Mokhonov, Vlad" uniqKey="Mokhonov V" first="Vlad" last="Mokhonov">Vlad Mokhonov</name></author><author><name sortKey="Safatov, Alexander S" sort="Safatov, Alexander S" uniqKey="Safatov A" first="Alexander S." last="Safatov">Alexander S. Safatov</name></author><author><name sortKey="Khromykh, Alexander A" sort="Khromykh, Alexander A" uniqKey="Khromykh A" first="Alexander A." last="Khromykh">Alexander A. Khromykh</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8780C3DFDC4C05963ECCC3E4A6AB4ED2B5174C6C</idno><date when="2007" year="2007">2007</date><idno type="doi">10.1111/j.1462-2920.2006.01226.x</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-GH98DX8D-W/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000832</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000832</idno><idno type="wicri:Area/Istex/Curation">000827</idno><idno type="wicri:Area/Istex/Checkpoint">000C52</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C52</idno><idno type="wicri:doubleKey">1462-2912:2007:Pyankov O:using:a:bioaerosol</idno><idno type="wicri:Area/Main/Merge">001F76</idno><idno type="wicri:Area/Main/Curation">001F75</idno><idno type="wicri:Area/Main/Exploration">001F75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Using a bioaerosol personal sampler in combination with real‐time PCR analysis for rapid detection of airborne viruses</title><author><name sortKey="Pyankov, Oleg V" sort="Pyankov, Oleg V" uniqKey="Pyankov O" first="Oleg V." last="Pyankov">Oleg V. Pyankov</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Environmental Sciences, Griffith University, Brisbane, 4111, Qld</wicri:regionArea><wicri:noRegion>Qld</wicri:noRegion></affiliation></author><author><name sortKey="Agranovski, Igor E" sort="Agranovski, Igor E" uniqKey="Agranovski I" first="Igor E." last="Agranovski">Igor E. Agranovski</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Environmental Sciences, Griffith University, Brisbane, 4111, Qld</wicri:regionArea><wicri:noRegion>Qld</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Australie</country></affiliation></author><author><name sortKey="Pyankova, Olga" sort="Pyankova, Olga" uniqKey="Pyankova O" first="Olga" last="Pyankova">Olga Pyankova</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, 4072, Qld</wicri:regionArea><wicri:noRegion>Qld</wicri:noRegion></affiliation></author><author><name sortKey="Mokhonova, Ekaterina" sort="Mokhonova, Ekaterina" uniqKey="Mokhonova E" first="Ekaterina" last="Mokhonova">Ekaterina Mokhonova</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, 4072, Qld</wicri:regionArea><wicri:noRegion>Qld</wicri:noRegion></affiliation></author><author><name sortKey="Mokhonov, Vlad" sort="Mokhonov, Vlad" uniqKey="Mokhonov V" first="Vlad" last="Mokhonov">Vlad Mokhonov</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, 4072, Qld</wicri:regionArea><wicri:noRegion>Qld</wicri:noRegion></affiliation></author><author><name sortKey="Safatov, Alexander S" sort="Safatov, Alexander S" uniqKey="Safatov A" first="Alexander S." last="Safatov">Alexander S. Safatov</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>State Research Center of Virology and Biotechnology ‘Vector’, Koltsovo, Novosibirsk Region, 630559</wicri:regionArea><wicri:noRegion>630559</wicri:noRegion></affiliation></author><author><name sortKey="Khromykh, Alexander A" sort="Khromykh, Alexander A" uniqKey="Khromykh A" first="Alexander A." last="Khromykh">Alexander A. Khromykh</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, 4072, Qld</wicri:regionArea><wicri:noRegion>Qld</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Environmental Microbiology</title><title level="j" type="alt">ENVIRONMENTAL MICROBIOLOGY</title><idno type="ISSN">1462-2912</idno><idno type="eISSN">1462-2920</idno><imprint><biblScope unit="vol">9</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="992">992</biblScope><biblScope unit="page" to="1000">1000</biblScope><biblScope unit="page-count">9</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2007-04">2007-04</date></imprint><idno type="ISSN">1462-2912</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1462-2912</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have recently developed a new personal sampler and demonstrated its feasibility for detection of viable airborne microorganisms including bacteria, fungi and viruses. To accelerate the time‐consuming analytical procedure involving 2–5 days of biological testing, we employed a real‐time PCR protocol in conjunction with the personal sampler for collection of airborne viruses. The advantage of this approach is that if the presence of a particular pathogen in the air is detected by the PCR, the remaining collecting liquid can be further analysed by more time‐consuming biological methods to estimate the number of airborne infectious/live microorganisms. As sampling of bioaerosols in natural environments is likely to be associated with substantial contamination by a range of microorganisms commonly existing in an ambient air, an investigation of the specificity of detection by targeted PCR analysis is required. Here we present the results of the study on the detection of Influenza virus in the ambient air contaminated with high concentrations of bacteria and fungi using real‐time PCR protocol. The combined sampling PCR detection method was found to be fully feasible for the rapid (∼2.5 h) and highly specific (no cross‐reactivity) identification of the labile airborne virus in the air containing elevated concentrations of other microorganisms.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Russie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Pyankov, Oleg V" sort="Pyankov, Oleg V" uniqKey="Pyankov O" first="Oleg V." last="Pyankov">Oleg V. Pyankov</name></noRegion><name sortKey="Agranovski, Igor E" sort="Agranovski, Igor E" uniqKey="Agranovski I" first="Igor E." last="Agranovski">Igor E. Agranovski</name><name sortKey="Agranovski, Igor E" sort="Agranovski, Igor E" uniqKey="Agranovski I" first="Igor E." last="Agranovski">Igor E. Agranovski</name><name sortKey="Khromykh, Alexander A" sort="Khromykh, Alexander A" uniqKey="Khromykh A" first="Alexander A." last="Khromykh">Alexander A. Khromykh</name><name sortKey="Mokhonov, Vlad" sort="Mokhonov, Vlad" uniqKey="Mokhonov V" first="Vlad" last="Mokhonov">Vlad Mokhonov</name><name sortKey="Mokhonova, Ekaterina" sort="Mokhonova, Ekaterina" uniqKey="Mokhonova E" first="Ekaterina" last="Mokhonova">Ekaterina Mokhonova</name><name sortKey="Pyankova, Olga" sort="Pyankova, Olga" uniqKey="Pyankova O" first="Olga" last="Pyankova">Olga Pyankova</name></country><country name="Russie"><noRegion><name sortKey="Safatov, Alexander S" sort="Safatov, Alexander S" uniqKey="Safatov A" first="Alexander S." last="Safatov">Alexander S. Safatov</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/StressCovidV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001F75 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001F75 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= StressCovidV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:8780C3DFDC4C05963ECCC3E4A6AB4ED2B5174C6C
|texte= Using a bioaerosol personal sampler in combination with real‐time PCR analysis for rapid detection of airborne viruses
}}
| This area was generated with Dilib version V0.6.33. |  |